The Regulation of Cell Size

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A Sigma factor gene which is important in the global regulation of heat shock responses in prokaryotes, rpoH, was isolated from Flexibacter chinesis by PCR, sequenced and compared to the rpoH genes of a variety of other organisms. The rpoH gene was 98% similar to other previously characterized genes. A mutant of rpoH was produced (JR102) which had a reduced growth rate at both low and high temp...

Cytotoxic effects of auraptene against a human malignant glioblastoma cell line

Objective: Glioblastoma multiforme (GBM) is the deadliest type of primary brain tumors, and the survival of patients is estimated to be only about one year. This study, for the first time, investigated the cytotoxic effects of auraptene on U87 GBM cell line.<b...

The Immunomodulatory Effect of Cyclophosphamide on Regulation of T-Cell Subsets and Antigen Presenting Cells.

Simultaneous regulation of miR-451 and miR-191 led to erythroid fate decision of mouse embryonic stem cell

Objective(s): Various microRNAs (miRNAs) are expressed during development of mammalian cells, when they aid in modulating gene expression by mediating mRNA transcript cleavage and/or regulation of translation rate. miR-191 and miR-451 have been shown to be critical regulators of hematopoiesis and have important roles in the induction of erythroid fate decision. So, the aim of this study is inve...

Epigallocatechin-3-Gallate Induces Apoptosis through Up-regulation of Bax and Down-regulation of Bcl-2 in Prostate Cancer Cell Line

Background and Aims: Epigallocatechin-3-gallate (EGCG) is a polyphenolic compound from green tea, which its anticancer effects on many types of cancers have been confirmed, but the molecular mechanism by which EGCG induces apoptosis remains unknown. The aim of the present study was to investigate anti-proliferative properties and apoptotic signaling pathway of EGCG on PC3 human prostate cancer ...

Knockdown of mitofilin inhibits autophagy and facilitates starvation-induced apoptosis in HeLa cells

Objective(s): Mitofilin contributes to the maintenance of mitochondrial structure and functions. This study was undertaken to determine the mechanisms underlying its regulation of apoptosis.  Materials and Methods: Mitofilin was knockdowned by specific short hairpin RNA (shRNA) and the stable HeLa cell clone was selected. The autophagy a...